rpoN Antibody

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Code	CSB-PA338580XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rpoN Polyclonal antibody

Uniprot No.

P24255

Target Names

rpoN

Alternative Names

rpoN antibody; glnF antibody; ntrA antibody; b3202 antibody; JW3169 antibody; RNA polymerase sigma-54 factor antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rpoN protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein GlnG(NtrC).

Gene References into Functions

DNA melting is an active process initiated in closed promoter complex (RPc) and that the RNAP conformations of intermediates are significantly different from that of RPc and open promoter complex. PMID: 28579332
Data suggest a direct binding mechanism, in which sigma(54)RNA polymerase (rpoN) reaches the local vicinity of promoters by 3D diffusion. PMID: 23720315
Results inidicate that there are 70 sigma(54) promoters in total in the entire Escherichia coli genome. PMID: 19969540
Identified as new member of the polyamine modulon together with Cra and H-NS. PMID: 17220219

Protein Families

Sigma-54 factor family

Database Links

KEGG: ecj:JW3169

STRING: 316385.ECDH10B_3376

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rpoN Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background