Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPB4 Polyclonal antibody

Uniprot No.

P20433

Target Names

RPB4

Alternative Names

RPB4 antibody; YJL140W antibody; J0654 antibody; DNA-directed RNA polymerase II subunit RPB4 antibody; RNA polymerase II subunit B4 antibody; B32 antibody; DNA-directed RNA polymerase II 32 kDa polypeptide antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPB4 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex is necessary for promoter-directed transcription initiation but is not required for recruitment of Pol II to active preinitiation complexes and seems to be dispensable for transcription elongation and termination. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. Involved in DNA repair of damage in the transcribed strand. RPB4 is dispensable under optimal growth conditions, but becomes essential during heat or cold shock and under nutrient depletion. Suppresses the RBP9-mediated transcription-coupled repair (TCR) subpathway of nucleotide excision repair (NER) but facilitates the RAD26-mediated TCR subpathway. Under stress conditions only, involved in mRNA export to the cytoplasm. Involved in mRNA decay. Promotes or enhances the deadenylation process of specific mRNAs and may recruit PAT1 and the LSM1-7 complex to these mRNAs, thus stimulating their decapping and further decay.

Gene References into Functions

Our data indicate that the environmental stress response that occurs in foot mutants depends mostly on a global post-transcriptional regulation mechanism which, in turn, depends on Rpb4-mRNA imprinting PMID: 27001033
Rpb4 is a bona fide Pol II core subunit that functions mainly in mRNA synthesis. PMID: 24802753
Rpb4 is required for elongation, in gene specific manner under certain conditions.Rpb4 assists Paf1 in elongation of FKS1 gene under cellular stress condition. PMID: 24780862
Subunit of RNA polymerase II, Rpb4p, is required for the decay of a class of mRNAs whose products are involved in protein synthesis. PMID: 16357218
Loss of Rpb4 reduces RNA polymerase II levels near 3' ends of multiple mRNA genes, decreases association of 3'-end processing factors, & alters polyadenylation site usage at the RNA14 gene. Rpb4 contributes to cotranscriptional 3'-end processing in vivo. PMID: 18195044
This report provides the first instance of a distinct role for Rpb4 in transcription, which is independent of its interacting partner, Rpb7. PMID: 18441121
Rpb4 is involved in the expression of genes downstream of the RAM pathway. PMID: 18687406

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, P-body. Note=Seems to shuttle between nucleus and cytoplasm in a complex with RPB7.

Protein Families

Eukaryotic RPB4 RNA polymerase subunit family

Database Links

KEGG: sce:YJL140W

STRING: 4932.YJL140W

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RPB4 Antibody

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