NRPB5A Antibody

Code CSB-PA110398XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPB5A Polyclonal antibody
Uniprot No.
Target Names
NRPB5A
Alternative Names
NRPB5A antibody; NRPD5A antibody; RPABC24.3 antibody; At3g22320 antibody; MCB17.4 antibody; MCB17_5 antibody; DNA-directed RNA polymerases II and IV subunit 5A antibody; RPB5a antibody; DNA-directed RNA polymerase II subunit E antibody; RNA polymerase I antibody; II and III 24.3 kDa subunit antibody; AtRPB24.3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRPB5A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. Component of RNA polymerase IV which mediates short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of target sequences.
Subcellular Location
Nucleus.
Protein Families
Archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family
Tissue Specificity
Expressed in roots, leaves and seeds, and to a lower level, in flower buds, flowers and siliques.
Database Links

KEGG: ath:AT3G22320

STRING: 3702.AT3G22320.1

UniGene: At.28063

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