Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPB7 Polyclonal antibody

Uniprot No.

P34087

Target Names

RPB7

Alternative Names

RPB7 antibody; YDR404C antibody; D9509.22 antibody; DNA-directed RNA polymerase II subunit RPB7 antibody; RNA polymerase II subunit B7 antibody; B16 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPB7 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II.

Gene References into Functions

Rpb4 and Rpb7 subunits dissociate from RNA polymerase II upon interaction with specific transcriptional elongation-associated proteins that are recruited to the hyperphosphorylated form of the C-terminal RNA polymerase II domain. PMID: 23418395
determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-A resolution PMID: 15591044
identified the regions that are involved in interaction between the Rpb4 and Rpb7 subunits PMID: 15913559
Rpb4p and Rpb7p shuttle via two pathways, depending on environmental conditions. PMID: 17056745
Rpb7p plays pivotal roles in determining mRNA levels. PMID: 17875743
Chromatin immunoprecipitation examined the role of the Rpb4/7 heterodimer in transcription in vivo in an rpb4Delta strain for effects on core polymerase & recruitment of other transcription factors. Rpb4/7 cross-links throughout transcribed regions. PMID: 18195044
the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle. PMID: 18667430
RPB4/RPB7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm. PMID: 18676807
growing RNA is leading toward Rpb4, not Rpb3, supporting the notion that RNA exits through the proposed pol II RNA exit channel 1 PMID: 19109435

Hide All

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, P-body. Note=Seems to shuttle between nucleus and cytoplasm in a complex with RPB4.

Protein Families

Eukaryotic RPB7/RPC8 RNA polymerase subunit family

Database Links

KEGG: sce:YDR404C

STRING: 4932.YDR404C

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RPB7 Antibody

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