Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPB9 Polyclonal antibody

Uniprot No.

P27999

Target Names

RPB9

Alternative Names

RPB9 antibody; YGL070C antibody; DNA-directed RNA polymerase II subunit RPB9 antibody; RNA polymerase II subunit B9 antibody; B12.6 antibody; DNA-directed RNA polymerase II 14.2 kDa polypeptide antibody; DNA-directed RNA polymerase II subunit 9 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPB9 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. Involved in the regulation of transcription elongation. Involved in DNA repair of damage in the transcribed strand. Mediates a transcription-coupled repair (TCR) subpathway of nucleotide excision repair (NER).

Gene References into Functions

conclude that Rpb9 supports intra-pol II interactions that modulate TL function and thus pol II enzymatic properties PMID: 27226557
Intrinsic transcript cleavage of Pol II requires the Rpb9 subunit. This enhances the backtrack recovery of Pol II. PMID: 26929337
Rpb9 plays an important role in maintaining transcriptional fidelity, whereas TFIIS may serve a different primary purpose PMID: 16492753
Rad26-mediated repair can be either transcription-coupled or transcription-independent; Rpb9-mediated repair is strictly transcription-coupled and is efficient only when the transcription level is high PMID: 17023424
The transcription elongation function of Rpb9 is involved in transcription-coupled DNA repair. PMID: 17030604
both S-II and Rpb9 maintain transcriptional fidelity by stimulating the cleavage activity intrinsic to RNA polymerase II PMID: 17535246

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Subcellular Location

Nucleus, nucleolus.

Protein Families

Archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family

Database Links

KEGG: sce:YGL070C

STRING: 4932.YGL070C

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RPB9 Antibody

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Target Background