Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) POLR3G Polyclonal antibody

Uniprot No.

O15318

Target Names

POLR3G

Alternative Names

DNA directed RNA polymerase III 32 kDa polypeptide antibody; DNA-directed RNA polymerase III subunit G antibody; DNA-directed RNA polymerase III subunit RPC7 antibody; Polr3g antibody; Polymerase (RNA) III (DNA directed) polypeptide G (32kD) antibody; RNA polymerase III 32 kDa subunit antibody; RNA polymerase III subunit C7 antibody; RPC32 antibody; RPC7 antibody; RPC7_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) POLR3G protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Gene References into Functions

POLR3G regulates small non-coding RNAs and RNA splicing in human pluripotent stem cells. The primary function of POLR3G is in the maintenance rather than repression of transcription. PMID: 28494942
Decreased levels of POLR3G result in loss of pluripotency and promote differentiation of human embryonic stem cells to all three germ layers but have no effect on cell apoptosis. PMID: 21898682

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Eukaryotic RPC7 RNA polymerase subunit family

Tissue Specificity

Barely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.

Database Links

HGNC: 30075

OMIM: 617456

KEGG: hsa:10622

STRING: 9606.ENSP00000382058

UniGene: Hs.282387

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POLR3G Antibody

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