RPN10 Antibody

Code CSB-PA183704XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPN10 Polyclonal antibody
Uniprot No.
Target Names
RPN10
Alternative Names
RPN10 antibody; MCB1 antibody; SUN1 antibody; YHR200W antibody; 26S proteasome regulatory subunit RPN10 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPN10 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Multiubiquitin binding protein.
Gene References into Functions
  1. Mutations in this disordered region promote ubiquitin chain extension of Rpn10. PMID: 26205498
  2. X-ray analysis revealed that these crystals of Rpn10 belonged to the monoclinic system C2, with unit-cell parameters a = 107.3, b = 49.7, c = 81.3 A, alpha = gamma = 90.0, beta = 130.5 degrees PMID: 22949210
  3. Data report that Rpn10 is monoubiquitinated in vivo and that this modification has profound effects on proteasome function. PMID: 20542005
  4. These findings underscore a broad role for Rpn10 in turnover of ubiquitylated substrates but a relatively modest role for its ubiquitin-binding UIM domain. PMID: 17644757
  5. These results suggest that Rpn10p is the receptor that binds the polyubiquitin chain during ubiquitin-dependent proteolysis of Gcn4p. PMID: 17978571
  6. Mutations in the hydrophobic core of ubiquitin affect its recognition by RPN10. PMID: 18054791
  7. The yeast homologue of S5a, Rpn10, enhanced the proteasomal degradation of troponin I to the same extent as S5a. PMID: 19387488

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Protein Families
Proteasome subunit S5A family
Database Links

KEGG: sce:YHR200W

STRING: 4932.YHR200W

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