rpnD Antibody

Code CSB-PA974038XA01EOG
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) rpnD Polyclonal antibody
Uniprot No.
Target Names
rpnD
Alternative Names
rpnD antibody; yjiP antibody; ECUMN_4949 antibody; Recombination-promoting nuclease RpnD antibody; EC 3.1.21.- antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Immunogen
Recombinant Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) rpnD protein
Immunogen Species
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
A low activity DNA endonuclease yielding 3'-hydroxyl ends. Expression of a repaired protein increases the frequency of recA-independent recombination, but also decreases viability probably via DNA damage as measured by induction of the SOS repair response; in a RecA strain expression has no effect on viability but does induce the SOS repair response. RecA-independent DNA recombination leads to replacement of recipient genes with large segments of donor DNA rather than DNA addition to the donor strain; increased expression of RpnD leads to smaller replacement segments, suggesting this protein may play a role in generating crossover events. Upon expression enhances RecA-independent DNA recombination 4.7-fold, concomitantly reducing viability by 94% and inducing DNA damage. May play a role in horizontal gene transfer.
Protein Families
Rpn/YhgA-like nuclease family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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