rpnD Antibody

Datasheet

Code	CSB-PA974038XA01EOG
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) rpnD Polyclonal antibody

Uniprot No.

B7NGZ6

Target Names

rpnD

Alternative Names

rpnD antibody; yjiP antibody; ECUMN_4949 antibody; Recombination-promoting nuclease RpnD antibody; EC 3.1.21.- antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)

Immunogen

A synthesized peptide derived from Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) rpnD protein

Immunogen Species

Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

A low activity DNA endonuclease yielding 3'-hydroxyl ends. Expression of a repaired protein increases the frequency of recA-independent recombination, but also decreases viability probably via DNA damage as measured by induction of the SOS repair response; in a RecA strain expression has no effect on viability but does induce the SOS repair response. RecA-independent DNA recombination leads to replacement of recipient genes with large segments of donor DNA rather than DNA addition to the donor strain; increased expression of RpnD leads to smaller replacement segments, suggesting this protein may play a role in generating crossover events. Upon expression enhances RecA-independent DNA recombination 4.7-fold, concomitantly reducing viability by 94% and inducing DNA damage. May play a role in horizontal gene transfer.

Protein Families

Rpn/YhgA-like nuclease family

Database Links

KEGG: eum:ECUMN_4949

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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