rpoA Antibody

Code CSB-PA364088XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rpoA Polyclonal antibody
Uniprot No.
Target Names
rpoA
Alternative Names
rpoA antibody; pez antibody; phs antibody; sez antibody; b3295 antibody; JW3257 antibody; DNA-directed RNA polymerase subunit alpha antibody; RNAP subunit alpha antibody; EC 2.7.7.6 antibody; RNA polymerase subunit alpha antibody; Transcriptase subunit alpha antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rpoA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.; Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNAP. It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis.
Gene References into Functions
  1. Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase has been described. PMID: 27729537
  2. Our investigations provide molecular insights for understanding how EctopoI interacts with RNAP, a critical step for preventing hypernegative DNA supercoiling during transcription PMID: 27448274
  3. Ribonucleoprotein particles of bacterial small non-coding RNA IsrA and its interaction with RNA polymerase core may link transcription to mRNA fate. PMID: 26609136
  4. Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator have been described. PMID: 26602687
  5. The authors report that the upstream-bound CRP interacts with thesealpha-CTD domains irrespective of whether it up- or downregulates promoter activity. PMID: 22967981
  6. A self-cleaving elastin-like polypeptide (ELP) tag was used to purify the multisubunit Escherichia coli RNA polymerase (RNAP) via a simple, nonchromatographic method. PMID: 20512976
  7. The direct interaction between RNAPalpha and RPL2 was confirmed both in vivo and in vitro by performing coimmunoprecipitation and bacterial two-hybrid experiments. PMID: 20097853
  8. carboxy-terminal domain of the alpha subunit of RNAP (alpha-CTD)contacts MarA at a surface distant from the DNA. PMID: 15458404
  9. Data show that SoxS diverts RNA polymerase alpha from UP-containing promoters to SoxS-activatable promoters PMID: 15465042
  10. Extensive stable DNA wrapping observed previously in the RNAP-promoter open complex at the lambda P(R) promoter is not a general feature of RNAP-promoter open complexes. PMID: 17290289
  11. Contacts between lambda repressor and the C-terminal domain of the RNA polymerase alpha subunit are required for efficient lambda repressor dependent activation of bacteriophage lambda pM promoter. PMID: 17389649

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Protein Families
RNA polymerase alpha chain family
Database Links
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