RPOT3 Antibody

Code CSB-PA522845XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPOT3 Polyclonal antibody
Uniprot No.
Target Names
RPOT3
Alternative Names
RPOT3 antibody; RPOPT antibody; RPOTP antibody; SCA3 antibody; At2g24120 antibody; F27D4.3 antibody; DNA-directed RNA polymerase 3 antibody; chloroplastic antibody; EC 2.7.7.6 antibody; Protein SCABRA 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RPOT3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Nuclear-encoded DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA in chloroplasts using the four ribonucleoside triphosphates as substrates. Required for chloroplast development and leaf mesophyll cell proliferation.
Gene References into Functions
  1. Exon 10 of the SCA3 gene contains 64-71 CAG repeats in all of the affected individuals. PMID: 24579313
  2. findings support a role for plastids in leaf morphogenesis and indicates that RpoTp is required for mesophyll cell proliferation PMID: 16698900
  3. evidence for nuclear-encoded RNA polymerases being represented by two phage-type RNA polymerases (RpoTp and RpoTmp) that have overlapping as well as gene-specific functions in the transcription of plastidial genes. PMID: 18084023
Subcellular Location
Plastid, chloroplast.
Protein Families
Phage and mitochondrial RNA polymerase family
Database Links

KEGG: ath:AT2G24120

STRING: 3702.AT2G24120.1

UniGene: At.21683

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