RPS2 Antibody

Code CSB-PA673716XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPS2 Polyclonal antibody
Uniprot No.
Target Names
RPS2
Alternative Names
RPS2 antibody; At4g26090 antibody; F20B18.200 antibody; Disease resistance protein RPS2 antibody; Resistance to Pseudomonas syringae protein 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RPS2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Disease resistance (R) protein that specifically recognizes the AvrRpt2 type III effector avirulence protein from Pseudomonas syringae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Acts via its interaction with RIN4, and probably triggers the plant resistance when RIN4 is degraded by AvrRpt2.
Gene References into Functions
  1. Rps2 segregates for two expressed clades of alleles, one resistant and one susceptible. Plants with resistant Rps2 are not less fit than those with a susceptible Rps2 allele in the absence of disease. Instead, all alleles provide a fitness benefit relative to an artificial deletion because of the role of RPS2 as a negative regulator of defence. PMID: 27428524
  2. Site-directed mutagenesis in the putative catalytic triad of AvrRpm1 results in loss of both AvrRpm1-dependent virulence and AvrRpm1-mediated activation of RPM1 but causes the ability to activate the RPS2 nucleotide binding leucine-rich repeat sensor. PMID: 22880057
  3. Tobacco rattle virus based virus-induce gene silencing which require RPS2-mediated resistance. PMID: 16845481
Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
Disease resistance NB-LRR family
Database Links

KEGG: ath:AT4G26090

STRING: 3702.AT4G26090.1

UniGene: At.46720

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