RPS5 Antibody

Datasheet

Code	CSB-PA526499XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPS5 Polyclonal antibody

Uniprot No.

O64973

Target Names

RPS5

Alternative Names

RPS5 antibody; At1g12220 antibody; T28K15.5 antibody; Disease resistance protein RPS5 antibody; Resistance to Pseudomonas syringae protein 5 antibody; pNd3/pNd10 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RPS5 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Disease resistance (R) protein that specifically recognizes the avrPphB type III effector avirulence protein from Pseudomonas syringae. Also confers resistance against Hyaloperonospora parasitica (downy mildew). Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Requires PBS1 to trigger the defense reaction against avrPphB. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1, suggesting that the cleavage of PBS1 could trigger an exchange of ADP for ATP, thereby activating RPS5. May function as a fine-tuned sensor of alterations in the structure of the effector target PBS1.

Gene References into Functions

involved in photosynthesis, plant development, and cold stress tolerance PMID: 27006483
RPS5 distinguishes PBS1 from closely related kinases based on a specific loop in the C-terminal half of PBS1. PMID: 24225654
the RPS5 polymorphism may not be maintained through a tightly coupled interaction involving a single coevolved R gene and effector pair; more likely, the stable polymorphism is maintained through complex and diffuse community-wide interactions PMID: 25043057
The RPS5 leucine-rich repeat domain functions to suppress RPS5 activation in the absence of PBS1 cleavage and promotes RPS5 activation in its presence. PMID: 22331412

Subcellular Location

Cell membrane; Lipid-anchor.

Protein Families

Disease resistance NB-LRR family

Database Links

KEGG: ath:AT1G12220

STRING: 3702.AT1G12220.1

UniGene: At.42084

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Organelles Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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RPS5 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background