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rpsJ Antibody

Datasheet
Code CSB-PA087644XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rpsJ Polyclonal antibody
Uniprot No.
P0A7R5
Target Names
rpsJ
Alternative Names
rpsJ antibody; nusE antibody; b3321 antibody; JW3283 antibody; 30S ribosomal protein S10 antibody; Small ribosomal subunit protein uS10 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rpsJ protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the binding of tRNA to the ribosomes. Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis. In complex with NusB is involved in the regulation of ribosomal RNA (rRNA) biosynthesis by transcriptional antitermination. S10 binds RNA non-specifically and increases the affinity of NusB for the boxA RNA sequence. S10 may constitute the critical antitermination component of the NusB-S10 complex.
Gene References into Functions
  1. NusG forms a complex with Rho or with NusE; because NusG amino-terminal domain contacts RNA polymerase & NusG carboxy-terminal domain interaction site of NusE is accessible in the ribosomal 30S subunit, NusG may be link between transcription & translation PMID: 20413501
Protein Families
Universal ribosomal protein uS10 family
Database Links
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