rseB Antibody

Code CSB-PA360340XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rseB Polyclonal antibody
Uniprot No.
Target Names
rseB
Alternative Names
rseB antibody; b2571 antibody; JW2555 antibody; Sigma-E factor regulatory protein RseB antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rseB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negatively modulates the activity of sigma-E (RpoE) by stabilizing RseA under non-stress conditions. Although not essential for association of sigma-E with Rsea it increases their affinity 2- to 3-fold. When bound to RseA it prevents proteolysis by DegS, which is probably relieved by lipopolysaccharide binding (LPS).
Gene References into Functions
  1. DegS and RseB are proposed to be stress sensor proteins for sigmaE signal transduction [review] PMID: 25935301
  2. Stress sensory protein RseB from E. coli, missing the periplasmic signal sequence, has been cloned, expressed, purified and crystallized. PMID: 16946473
  3. Results suggest that RseB acts as a sensor of periplasmic stress with a dual functionality: it detects mislocalized lipoproteins and propagates the signal to induce the sigma(E)-response. PMID: 17692869
Subcellular Location
Periplasm. Note=Partially associates with the inner membrane via RseA.
Protein Families
RseB family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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