Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rsgA Polyclonal antibody

Uniprot No.

P39286

Target Names

rsgA

Alternative Names

rsgA antibody; engC antibody; yjeQ antibody; b4161 antibody; JW4122 antibody; Small ribosomal subunit biogenesis GTPase RsgA antibody; EC 3.6.1.- antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rsgA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Binds the 30S subunit contacting the head, platform, and rRNA helix 44, which may assist the last maturation stages. Removes RbfA from mature, but not immature 30S ribosomes in a GTP-dependent manner; 95% removal in the presence of GTP, 90% removal in GMP-PNP and 65% removal in the presence of GDP. Circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96-fold increase in kcat) ribosomes. Mature 30S ribosomes stimulate intrinsic GTPase more than do immature 30S ribosomes. Ribosome-associated GTPase activity is stimulated by RbfA. The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin streptomycin and spectinomycin. This inhibition is not due to competition for binding sites on the 30S or 70S ribosome.

Gene References into Functions

This finding indicates that, along with acting as an assembly factor, YjeQ has a role as a checkpoint protein, consisting of testing the proofreading ability of the 30S subunit. The structure also informs the mechanism by which YjeQ implements the release from the 30S subunit of a second assembly factor, called RbfA. PMID: 28396444
costructure revealed that YjeQ interacts simultaneously with helix 44, the head and the platform of the 30S subunit PMID: 21960487
RsgA may be close contact with the A site of the decoding center of the small subunit of the ribosome. PMID: 18029743
The work is most consistent with the hypothesis that YjeQ has a role in late 30S subunit biogenesis. PMID: 18223068
Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. PMID: 19620234

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Subcellular Location

Cytoplasm.

Protein Families

TRAFAC class YlqF/YawG GTPase family, RsgA subfamily

Database Links

KEGG: ecj:JW4122

STRING: 316385.ECDH10B_4356

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rsgA Antibody

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