rsmE Antibody

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Code	CSB-PA313443XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rsmE Polyclonal antibody

Uniprot No.

P0AGL7

Target Names

rsmE

Alternative Names

rsmE antibody; yggJ antibody; b2946 antibody; JW2913 antibody; Ribosomal RNA small subunit methyltransferase E antibody; EC 2.1.1.193 antibody; 16S rRNA m3U1498 methyltransferase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) rsmE protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.

Gene References into Functions

both crystal and solution structures of E. coli RsmE were studied; study provides new information on the structure-function relationship of RsmE and thereby suggests a novel catalytic mechanism PMID: 22925577
The protein encoded by yggJ, a member of the uncharacterized DUF558 protein family of predicted alpha/beta (trefoil) knot methyltransferases is responsible for methylation at U1498 in 16S rRNA. PMID: 16431987
RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation, and that methylation events in the 3' minor domain of 16S rRNA probably occur late during 30S ribosome assembly. PMID: 17872509

Subcellular Location

Cytoplasm.

Protein Families

RNA methyltransferase RsmE family

Database Links

KEGG: ecj:JW2913

STRING: 316385.ECDH10B_3121

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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rsmE Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background