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rsmE Antibody

Datasheet
Code CSB-PA313443XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rsmE Polyclonal antibody
Uniprot No.
P0AGL7
Target Names
rsmE
Alternative Names
rsmE antibody; yggJ antibody; b2946 antibody; JW2913 antibody; Ribosomal RNA small subunit methyltransferase E antibody; EC 2.1.1.193 antibody; 16S rRNA m3U1498 methyltransferase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rsmE protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.
Gene References into Functions
  1. both crystal and solution structures of E. coli RsmE were studied; study provides new information on the structure-function relationship of RsmE and thereby suggests a novel catalytic mechanism PMID: 22925577
  2. The protein encoded by yggJ, a member of the uncharacterized DUF558 protein family of predicted alpha/beta (trefoil) knot methyltransferases is responsible for methylation at U1498 in 16S rRNA. PMID: 16431987
  3. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation, and that methylation events in the 3' minor domain of 16S rRNA probably occur late during 30S ribosome assembly. PMID: 17872509
Subcellular Location
Cytoplasm.
Protein Families
RNA methyltransferase RsmE family
Database Links
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