NEDP1 Antibody

Code CSB-PA881803XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NEDP1 Polyclonal antibody
Uniprot No.
Target Names
NEDP1
Alternative Names
NEDP1 antibody; DEN1 antibody; At5g60190 antibody; F15L12.8 antibody; NEDD8-specific protease 1 antibody; EC 3.4.22.- antibody; Deneddylase-1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NEDP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Processes the pre-form of the ubiquitin-like protein NEDD8/RUB1. Has the capacity to discriminate between NEDD8/RUB1 and ubiquitin. Has no SUMO protease activity.
Gene References into Functions
  1. DENEDDYLASE1 functions to maintain NEDD8 pathway activity by recovering diverted NEDD8 moieties from proteins of the NEDD8 conjugation machinery. [DEN1] PMID: 28096463
  2. mutants of a DEN1 homolog from Arabidopsis thaliana have no detectable defects in NEDD8 processing but do accumulate a broad range of NEDD8 conjugates. Neddylation may serve as a regulatory mechanism for cullin and non-cullin proteins. [DEN1] PMID: 25783028
Protein Families
Peptidase C48 family
Database Links

KEGG: ath:AT5G60190

STRING: 3702.AT5G60190.1

UniGene: At.49827

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