rumi Antibody

Code CSB-PA823424XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) rumi Polyclonal antibody
Uniprot No.
Target Names
rumi
Alternative Names
rumi antibody; CG31152 antibody; O-glucosyltransferase rumi antibody; EC 2.4.1.- antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) rumi protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue found in the consensus sequence C-X-S-X-[PA]-C in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.
Gene References into Functions
  1. The study suggests that loss of Rumi activity may underlie these diseases, and the mechanistic insights may facilitate the development of modulators of Notch signaling. PMID: 27428513
  2. our data suggest that O-glucosylation of Eys by Rumi ensures rhabdomere separation by promoting proper Eys folding and stability in a critical time window during the mid-pupal stage. PMID: 25412384
  3. Rumi functions as both a protein O-glucosyltransferase and a protein O-xylosyltransferase. PMID: 21949356
  4. Rumi is a CAP10 domain glycerosyltransferase that modifies Notch and is required for Notch signaling. PMID: 18243100
Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Glycosyltransferase 90 family
Database Links

KEGG: dme:Dmel_CG31152

STRING: 7227.FBpp0083713

UniGene: Dm.1286

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