Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Ruvbl2 Polyclonal antibody

Uniprot No.

Q9WTM5

Target Names

Ruvbl2

Alternative Names

Ruvbl2 antibody; RuvB-like 2 antibody; EC 3.6.4.12 antibody; p47 protein antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Ruvbl2 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes. May play a role in regulating the composition of the U5 snRNP complex.

Gene References into Functions

Ruvbl2 has a role in the differentiation of neuroectoderm during early embryogenesis. PMID: 27469992
accumulating E2f1 progressively recruits a Pontin/Reptin complex to open the chromatin conformation at E2f target genes and amplifies the E2f transcriptional response. PMID: 26639898
Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression. PMID: 24167278
We demonstrate that leukemogenic activity of MLL-AF9 requires RUVBL2 (RuvB-like 2), an AAA+ ATPase family member that functions in a wide range of cellular processes, including chromatin remodeling and transcriptional regulation. PMID: 23403462
A forward genetic screen reveals roles for Nfkbid, Zeb1, and Ruvbl2 in humoral immunity. PMID: 22761313
Ectopic expression of RUVBL2 decreases the levels of ARF, whereas knockdown of RUVBL2 results in a marked increase in ARF levels. In addition, RUVBL2 down-regulates the levels of p53 in an ARF-dependent manner. PMID: 22285491
Reptin, a chromatin-remodeling factor, is methylated at lysine 67 in hypoxic conditions by the methyltransferase G9a. PMID: 20603076
RuvBl2, which encodes an ATP-dependent DNA helicase, is specifically detected in the spermatocytes and spermatids of the adult testis as well as in primordial germ cells. PMID: 16425234
RUVBL2 plays an important role in insulin-stimulated GLUT4 translocation through its interaction with AS160 PMID: 19532121

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Subcellular Location

Nucleus. Dynein axonemal particle.

Protein Families

RuvB family

Database Links

KEGG: mmu:20174

STRING: 10090.ENSMUSP00000033087

UniGene: Mm.34410

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Ruvbl2 Antibody

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