RVS167 Antibody

Code CSB-PA334610XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RVS167 Polyclonal antibody
Uniprot No.
Target Names
RVS167
Alternative Names
RVS167 antibody; YDR388W antibody; D9509.8 antibody; Reduced viability upon starvation protein 167 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RVS167 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of a cytoskeletal structure that is required for the formation of endocytic vesicles at the plasma membrane level. Could be implicated in cytoskeletal reorganization in response to environmental stresses and could act in the budding site selection mechanism.
Gene References into Functions
  1. Genetic, bioinformatics, biochemical and biophysical data show that attraction between this alpha1-conjugated ubiquitin and the HECT ubiquitin-binding patch pulls the alpha1-helix out of the interface, thereby promoting trimerization. Strikingly, trimerization renders the ubiquitin ligase inactive. PMID: 28069708
  2. deletion of RVS167 causes downregulation of de novo sphingolipid biosynthesis via Orm2 PMID: 27312128
  3. reveal a conserved role for calmodulin in regulating the intrinsic membrane-sculpting activity of endocytic N-BAR domains, Calmodulin-binding mutants of Rvs167 exhibited defects in endocytic vesicle release PMID: 27093085
  4. 59 of 131 in: PLoS One. 2013;8(2) (dup#16) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis PMID: 23469085
  5. Vps1 and Rvs167 function together to mediate scission during endocytosis in Saccharomyces cerevisiae. PMID: 22082017
  6. In gyp5Deltagyl1Delta cells, Rvs167p is not correctly localized to the small-bud tip. PMID: 21554509

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Subcellular Location
Cytoplasm, cytoskeleton.
Database Links

KEGG: sce:YDR388W

STRING: 4932.YDR388W

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