SAP130A Antibody

Code CSB-PA109141XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SAP130A Polyclonal antibody
Uniprot No.
Target Names
SAP130A
Alternative Names
SAP130A antibody; At3g55200 antibody; T26I12.80 antibody; Spliceosome-associated protein 130 A antibody; AtSAP130a antibody; SAP 130 A antibody; Pre-mRNA-splicing factor SF3b 130 kDa subunit A antibody; SF3b130 A antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SAP130A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Required for pollen and ovule development, especially during the transition from microspore to the bicellular stage in pollen development. Involved in the accumulation of QRT1 and QRT3.
Gene References into Functions
  1. AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction.[AtSAp130a] PMID: 21680607
Subcellular Location
Nucleus.
Protein Families
RSE1 family
Tissue Specificity
Expressed at low levels in roots, leaves, inflorescence and, to a lower extent, in siliques.
Database Links

KEGG: ath:AT3G55200

UniGene: At.28226

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