Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SAC1 Polyclonal antibody

Uniprot No.

P32368

Target Names

SAC1

Alternative Names

SAC1 antibody; RSD1 antibody; YKL212W antibody; Phosphoinositide phosphatase SAC1 antibody; EC 3.1.3.- antibody; Recessive suppressor of secretory defect antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SAC1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Phosphoinositide phosphatase which catalyzes the hydrolysis of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 4-phosphate (PtdIns(4)P). Has low activity towards phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). May be involved in the coordination of the activities of the secretory pathway and the actin cytoskeleton.

Gene References into Functions

this study showed that the PI4P-phosphatase Sac1p, by maintaining a PI4P gradient at the endoplasmic reticulum / plasma membrane interface, drove phosphatidylserine transport. PMID: 26206936
Collectively, these results suggested that Sac1 is involved in the maintenance of a normal rate of biosynthesis and distribution of phosphatidylserine. PMID: 24578286
Sac1 trafficking depends on Hog1 function. PMID: 22882253
Vps74 binds directly to the catalytic domain of Sac1 PMID: 22553352
lipid composition of the substrate membrane plays an important role in the control of Sac1 function PMID: 22452743
A COOH-terminal region in yeast Sac1p is crucial for ER targeting by directly interacting with dolicholphosphate mannose synthase Dpm1p. PMID: 15657391
analysis of growth control of Golgi phosphoinositides by reciprocal localization of sac1 lipid phosphatase and pik1 4-kinase PMID: 17908202
Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P PMID: 18226253
Modulation of sphingolipid metabolism by the phosphatidylinositol-4-phosphate phosphatase Sac1p through regulation of phosphatidylinositol in Saccharomyces cerevisiae PMID: 19139096

Hide All

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.

Database Links

KEGG: sce:YKL212W

STRING: 4932.YKL212W

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA333690XA01SVG
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

SAC1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background