SAC3 Antibody

Code CSB-PA341633XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SAC3 Polyclonal antibody
Uniprot No.
Target Names
SAC3
Alternative Names
SAC3 antibody; LEP1 antibody; YDR159W antibody; YD8358.13 antibody; Nuclear mRNA export protein SAC3 antibody; Leucine permease transcriptional regulator antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SAC3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket.
Gene References into Functions
  1. In the TREX-2 complex the TPR-like repeat region of the Sac3 chain extends further towards the N-terminus than had been previously thought. PMID: 27422657
  2. Study finds that the TREX-2 complex (1) interacts with the Mediator Med31 submodule via the Sac3 PCI domain, (2) regulates Mediator association with the Cdk8 kinase, (3) impacts on Pol II carboxyl terminus domain Ser5 phosphorylation, and (4) employs a similar, conserved Sac3 PCI domain surface to promote transcription and mRNA export. PMID: 26317468
  3. Data show that the Sus1-binding site on Sgf11 is somewhat shorter than on Sac3 and is based on a narrower hydrophobic stripe. PMID: 20007317
  4. Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process PMID: 18667528
Subcellular Location
Nucleus envelope. Note=Localizes to the nuclear pores.
Protein Families
SAC3 family
Database Links

KEGG: sce:YDR159W

STRING: 4932.YDR159W

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