SAD2 Antibody

Datasheet

Code	CSB-PA276141XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SAD2 Polyclonal antibody

Uniprot No.

F4IRR2

Target Names

SAD2

Alternative Names

SAD2 antibody; EMA1 antibody; URM9 antibody; At2g31660 antibody; Importin beta-like SAD2 antibody; Protein ENHANCED MIRNA ACTIVITY 1 antibody; Protein SUPER SENSITIVE TO ABA AND DROUGHT 2 antibody; Protein UNARMED 9 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SAD2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Functions probably in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with other importin subunits (Probable). Involved in the regulation of the abscisic acid (ABA)-mediated pathway in response to cold or salt stress. Involved in UV-B responses by regulating accumulation of UV-absorbing pigments through mediation of MYB4 nuclear transport. Involved in trichome initiation by controlling GL1, GL2, GL3 and TTG1 transcription and may affect an upstream regulator of GL3 and disrupt complex function. Acts as negative regulator miRNA activity by regulating miRNA loading into AGO1 complexes.

Gene References into Functions

EMA1 modulates miRNA activity by influencing the loading of miRNAs into AGO1 complexes. These results implicate EMA1 as a negative regulator of the miRNA pathway and reveal a novel layer of miRNA activity modulation. PMID: 21984696
SAD2 plays a specific role in nuclear transport in ABA signaling. [SAD2] PMID: 16889648
Arabidopsis thaliana mutant sensitive to ABA and drought2 (sad2), which harbors a T-DNA insertion in an importin beta-like gene, is more tolerant to UV-B radiation than the wild type. PMID: 17993626
Expression levels of GL1, MYB23, GL2 and TTG1 were reduced in shoots of sad2 mutants while expression levels of GL3 and ENHANCER OF GLABRA3 (EGL3) were enhanced. PMID: 18713401

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Importin beta family

Tissue Specificity

Expressed in roots, epidermal and guard cells of leaves, stems and siliques.

Database Links

KEGG: ath:AT2G31660

STRING: 3702.AT2G31660.1

UniGene: At.46140

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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