sav Antibody

Code CSB-PA893258XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) sav Polyclonal antibody
Uniprot No.
Target Names
sav
Alternative Names
sav antibody; SHRP antibody; CG33193 antibody; Scaffold protein salvador antibody; Shar-pei antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) sav protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Required for cell cycle exit in eye imaginal disk and hid-induced apoptotic cell deaths that are part of normal retinal development. Activation of Drice in eye imaginal disk by either Hid or Rpr is almost completely blocked by Sav expression.
Gene References into Functions
  1. Serine-17 is a key residue for the regulation of Sav stability. PMID: 23524264
  2. The Salvador pathway restrains tissue growth by phosphorylating and inactivating the oncoprotein Yorkie. PMID: 22075147
  3. The salvador protein pathway restrains tissue growth by phosphorylating and inactivating the oncoprotein Yorkie. PMID: 22075148
  4. a gene, salvador, which is especially significant, because it not only regulates and coordinates both exit from the cell cycle and apoptosis, but also has a human homolog that may play a key role in tumorigenesis. PMID: 12242148
  5. Data show that salvador genes are dispensable in germline cells for their proliferation control. PMID: 18095349

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Tissue Specificity
Third instar larvae eye disk, expressed in a stripe in the morphogenetic furrow, decreases in the region of the second mitotic wave (SMW) and increases once again posterior to the SMW.
Database Links

KEGG: dme:Dmel_CG33193

STRING: 7227.FBpp0083749

UniGene: Dm.1289

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