sbcD Antibody

Code CSB-PA313428XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) sbcD Polyclonal antibody
Uniprot No.
Target Names
sbcD
Alternative Names
sbcD antibody; yajA antibody; b0398 antibody; JW0388 antibody; Nuclease SbcCD subunit D antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) sbcD protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.
Gene References into Functions
  1. Data show that replication forks form a palindrome-like structural intermediate that requires nucleolytic processing by SbcC-SbcD and exonuclease I (ExoI) before chromosome replication can be completed. PMID: 29208713
  2. The results suggest that SbcCD processes hairpin and linear dsDNA ends with a novel DNA end-dependent binary endonuclease activity in response to substrate length. PMID: 26319016
  3. The SbcD monomer exhibits single-stranded DNA endonuclease activity and double-stranded DNA exonuclease activity. PMID: 24531464
  4. In sbcCD mutants, RecA4142 can bind other DNA substrates by itself that are normally degraded by the SbcCD nuclease. PMID: 20304994
  5. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein, and SbcD protein is distributed through the cytoplasm. PMID: 17644583
  6. The absence of SbcC and SbcD in sbcB15 cells decreased stationary-phase mutation. PMID: 19018518
  7. Study found that functions of SbcCD, a DNA structure specific nuclease, were needed in the RNase E-dependent lacZ expression of the GAA repeats. PMID: 19733517

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Protein Families
SbcD family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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