MCD1 Antibody

1. budding yeast Plk1, Cdc5, is pre-deposited onto cohesin engaged in cohesion on chromosome arms in G2/M phase cells. The Cdc5-cohesin association is mediated by direct interaction between the polo-box domain of Cdc5 and Scc1 phosphorylated at multiple sites in its middle region. PMID: 27325700
2. the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability. PMID: 26923589
3. Study shows that cohesin's release from the DNA is mediated by disengagement of the Smc3/Scc1 interface involving interaction between the SMC ATPase heads and is inhibited by Smc3 acetylation. PMID: 26895425
4. Studied the function of Mcd1 in meiosis by ectopically expressing Mcd1 during early meiotic prophase I in Saccharomyces cerevisiae. PMID: 25737120
5. We show that residues Y371 and K372 within the SCD are critical for viability and chromosome condensation but dispensable for cohesion. However, scc3 Y371A and scc3 K372A bind normally to Mcd1 PMID: 25748820
6. In the linker region of Mcd1p, we identified a novel evolutionarily conserved 10-amino acid cluster, termed the regulation of cohesion and condensation (ROCC) box. The ROCC box is required for the establishment of condensation. PMID: 24966169
7. Expression of SCC1 is both necessary and sufficient for the binding of cohesin to its loader, the association of Scc2 with centromeres, and cohesin loading. PMID: 23499533
8. a separase-resistant allele of the gene coding for the alpha-kleisin subunit of cohesin, Mcd1 (also known as Scc1), reduces double-strand break resection and compromises the efficiency of repair even when loaded during DNA damage PMID: 23178808
9. SUMOylation of Mcd1 is a novel prerequisite for the establishment of DNA damage-induced cohesion at DSB-proximal regions and cohesion-associating regions (CARs) genome-wide. PMID: 22771042
10. The expression of MCD1 was not subject to Scc3 regulation in vegetative cells. PMID: 21270391
11. cleavage of chromatin-bound cohesin subunit SCC1 after targeted phosphorylation by Polo-like kinase PMID: 15241476
12. We provide molecular evidence that cohesins are required for repair of replication-born DSBs by SCE, as determined in smc3, scc1 and scc2 mutants, but not for other recombinational repair events. PMID: 16888651
13. Scc1 connects the Smc1 and Smc3 ATPase heads and regulates their ATPase activity. PMID: 17055978
14. The activated Esp1 acts as caspase-like protease for the cleavage of Mcd1, which enhances the cell death via its translocation from nucleus to mitochondria. PMID: 18321989
15. Double-strand breaks-induced cohesion occurs when cohesin contains the kleisin subunit, Mcd1 (Scc1), but not when Mcd1 is replaced by its meiotic isoform, Rec8. PMID: 18614046

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KEGG: sce:YDL003W

STRING: 4932.YDL003W