SCM3 Antibody

Code CSB-PA623609XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SCM3 Polyclonal antibody
Uniprot No.
Target Names
SCM3
Alternative Names
SCM3 antibody; YDL139C antibody; D2155 antibody; Protein SCM3 antibody; Suppressor of chromosome missegregation protein 3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SCM3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Gene References into Functions
  1. Cse4 and H3 are structurally compatible to be incorporated in the same nucleosome to form heterotypic particles during Scm3-mediated nucleosome assembly. PMID: 24623811
  2. Scm3 becomes 2.5-fold enriched at kinetochores in anaphase after incorporation of Ces4 and then dissociated from kinetochores with mitotic exit. PMID: 21926480
  3. stringent regulation of HJURP and SCM3 expression are critical for genome stability. PMID: 21980305
  4. Scm3 functions as a Cse4-specific nucleosome assembly factor, and the resulting octameric nucleosomes do not contain Scm3 as a stably bound component. PMID: 21587230
  5. Study propose that Scm3 plays an active role in centromeric nucleosome assembly. PMID: 21317428
  6. Ndc10/Scm3 binds to centromeric DNA, which is in turn essential for targeting Cse4 to centromeres. PMID: 17569568
  7. nonhistone Scm3 serves to assemble and maintain Cse4-H4 at centromeres and may replace histone H2A-H2B dimers in a centromere-specific nucleosome core PMID: 17574026

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YDL139C

STRING: 4932.YDL139C

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