SCY2 Antibody

Code CSB-PA520716XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SCY2 Polyclonal antibody
Uniprot No.
Target Names
SCY2
Alternative Names
SCY2 antibody; EMB2289 antibody; At2g31530 antibody; T9H9.5 antibody; Preprotein translocase subunit SCY2 antibody; chloroplastic antibody; Protein EMBRYO DEFECTIVE 2289 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SCY2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in protein export. Probably interacts with other proteins to allow the postimport or conservative sorting pathway for inner membrane proteins in plastids. Central subunit of the protein translocation channel SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug.
Gene References into Functions
  1. The region of SCY2 that contains transmembrane domains 3 and 4 is necessary for localization to the inner envelope and may serve as a membrane anchor, enhancing the integration of other transmembrane domains via either stop-transfer or post-import mechanisms. PMID: 28992187
  2. Data show that the SEC2 translocase consisting of SCY2, SECE2, and SECA2 likely integrates a subset of inner envelope membrane proteins. PMID: 28213560
  3. Data indicate that SECE2 plays an essential role in plant growth and development, and specifically interacts with the SCY2 component in vivo. PMID: 26406904
  4. SCY2 and SECA2 function in Sec-mediated integration and translocation processes at the inner envelope membrane. PMID: 21051552
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein. Plastid, amyloplast membrane; Multi-pass membrane protein. Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein. Note=Predominantly localized to envelope membranes.
Protein Families
SecY/SEC61-alpha family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT2G31530

STRING: 3702.AT2G31530.1

UniGene: At.27291

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