sdiA Antibody

Code CSB-PA357001XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) sdiA Polyclonal antibody
Uniprot No.
Target Names
sdiA
Alternative Names
sdiA antibody; b1916 antibody; JW1901 antibody; Regulatory protein SdiA antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) sdiA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Activates cell division by specifically increasing transcription from one of the two promoters that lie immediately upstream of the ftsQAZ gene cluster. Activates ydiV expression in response to extracellular autoinducer AI-1 (Vibrio fischeri autoinducer oxoC6).
Gene References into Functions
  1. the characterization of a set of small, regulatory RNAs (sRNAs) expressed from an Escherichia coli locus we have denoted sdsN located adjacent to the LuxR-homolog gene sdiA, is reported. PMID: 27166377
  2. SdiA interacts with multiple species of effector ligands and its promoter recognition specificity is modulated to various directions depending on the interacting effectors. PMID: 24645791
  3. Crystal structures of intact SdiA are reported; the E. coli quorum-sensing receptor SdiA seems to bridge both intraspecies and interspecies communication. PMID: 24598739
  4. SdiA does not respond to indole but indole did inhibit N-acyl-l-homoserine lactone sensing by SdiA. PMID: 22610437
  5. CsrA represses translation of sdiA by binding to the coding region of sdiA mRNA. PMID: 21908661
  6. roles of SdiA in E. coli (and Salmonella typhimurium): cell division; antibiotic resistance; virulence; biofilms; acid resistance; gene expression regulation; microbial viability [sdiA; quorum-sensing transcriptional activator; [REVIEW] PMID: 21034261
  7. NMR analysis of assignments for Escherichia coli SdiA1-171 PMID: 15929010
  8. The three-dimensional structure of a complex between the N-terminal domain of SdiA and a candidate autoinducer N-octanoyl-L-homoserine lactone (C8-HSL) has been calculated in solution from NMR data. PMID: 16307757
  9. Wild-type SdiA decreased biofilm formation by reducing curli production and motility. PMID: 19168658

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