SDN1 Antibody

Code CSB-PA385279XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SDN1 Polyclonal antibody
Uniprot No.
Target Names
SDN1
Alternative Names
SDN1 antibody; At3g50100 antibody; F3A4.180 antibody; Small RNA degrading nuclease 1 antibody; EC 3.1.-.- antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SDN1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
3'-5' exonuclease degrading single-stranded small RNAs.
Gene References into Functions
  1. The SDN1 C-terminal domain adopts an RNA recognition motif fold and is critical for substrate binding and enzymatic processivity of SDN1.SDN1 binds AGO protein PAZ domain and trims the 3' ends of AGO-bound miRNAs. PMID: 30181559
  2. AGO10 reduces miR165/6 accumulation by enhancing its degradation by SDN1 and SDN2 in vivo. PMID: 28231321
  3. findings show that a family of exoribonucleases encoded by SMALL RNA DEGRADING NUCLEASE genes degrades mature miRNAs; SDN1 acts specifically on single-stranded miRNAs in vitro & is sensitive to 2'-O-methyl modification on the 3' terminal ribose of miRNAs PMID: 18787168
Subcellular Location
Nucleus.
Protein Families
REXO1/REXO3 family
Database Links

KEGG: ath:AT3G50100

STRING: 3702.AT3G50100.1

UniGene: At.35533

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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