sdpC Antibody

Datasheet

Code	CSB-PA520198XA01BRJ
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Product Details

Full Product Name

Rabbit anti-Bacillus subtilis (strain 168) sdpC Polyclonal antibody

Uniprot No.

O34344

Target Names

sdpC

Alternative Names

sdpC antibody; yvaY antibody; BSU33770 antibody; Sporulation delaying protein C antibody; SdpC antibody; Cannibalism toxin SDP antibody; Killing factor SdpC antibody; Toxic peptide SdpC) [Cleaved into: Sporulation delaying protein antibody; SDP)] antibody

Raised in

Rabbit

Species Reactivity

Bacillus subtilis (strain 168)

Immunogen

Recombinant Bacillus subtilis (strain 168) sdpC protein

Immunogen Species

Bacillus subtilis (strain 168)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Produces a 42-residue extracellular sporulation delaying protein (SDP) that collapses the proton motive force (probably both the membrane potential and pH gradient) across the cell membrane, which leads to autolysis; may form a proton channel. Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, inducing cannibalism to provide a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation. Addition of SDP to liquid cultures halts growth, leads to increased cell permeability and eventually cell lysis in a significant subset of the population, although some cells survive and resume growth after a lag period. Effects of SDP are irreversible within 10 minutes. Addition of SDP to solid cultures induces killing, it is much more effective than SKF (AC O31422). Has antibiotic action against Gram-positive Firmicutes (L.acidophilus, M.megaterium, P.polymyxa, S.aureus, S.epidermidis) but not Actinobacteria M.luteus or Gram-negative P.aeruginosa or K.pneumoniae. SDP induces expression of the sdpR-sdpI operon. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, binds to SdpI triggering a signal transduction cascade that protects the cell against the toxic effects of its own SDP.

Gene References into Functions

These data suggest that SDP toxin production is a multistep process and that SdpAB are required for SDP production likely by controlling, directly or indirectly, cleavage of SDP from the pro-SdpC precursor. PMID: 23687264

Subcellular Location

Secreted. Note=Produces a secreted protein originating from this gene (PubMed:12817086), secreted by the general secretory pathway (PubMed:14568161).

Database Links

KEGG: bsu:BSU33770

STRING: 224308.Bsubs1_010100018321

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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sdpC Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background