SEC Antibody

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Code	CSB-PA868144XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SEC Polyclonal antibody

Uniprot No.

Q9M8Y0

Target Names

SEC

Alternative Names

SEC antibody; At3g04240 antibody; T6K12.14 antibody; Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC antibody; EC 2.4.1.255 antibody; Protein SECRET AGENT antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SEC protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

O-linked N-acetylglucosamine transferase (OGT) that mediates O-glycosylation of capsid protein (CP) of virus in case of infection by Plum pox virus. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Its OGT activity has been proved in vitro but not in vivo. Required with SPY for gamete and seed development. Activates ATX1 through O-GlcNAc modification to augment ATX1-mediated H3K4me3 histone epigenetic modification at FLC locus, thus preventing premature flowering.

Gene References into Functions

DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. PMID: 26773002
SECRET AGENT (SEC) functions in germination, rosette leaf growth, height of inflorescence stem, and the rate of production of rosette leaves. PMID: 16473894

Protein Families

Glycosyltransferase 41 family, O-GlcNAc transferase subfamily

Database Links

KEGG: ath:AT3G04240

STRING: 3702.AT3G04240.1

UniGene: At.27850

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SEC Antibody

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