SEC24 Antibody

Code CSB-PA331178XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC24 Polyclonal antibody
Uniprot No.
Target Names
SEC24
Alternative Names
SEC24 antibody; ANU1 antibody; YIL109C antibody; Protein transport protein SEC24 antibody; Abnormal nuclear morphology 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC24 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. SEC24 specifically recruits cargo proteins like BET1 or SYS1 to the COPII vesicles. The SEC23/24 complex is also involved in internalization of plasma membrane proteins like the maltose transporter.
Gene References into Functions
  1. Here the authors show that phosphorylation of conserved amino acids on the membrane-distal surface of the Saccharomyces cerevisiae COPII cargo adaptor, Sec24, reprograms COPII vesicles for autophagy. PMID: 27855785
  2. Distribution of Sec24 isoforms to each ER exit site is dynamically regulated in Saccharomyces cerevisiae. PMID: 25896017
  3. association of Sec24p with Sec16p creates a regulatory complex that retards the GTPase activity of the COPII coat to prevent premature vesicle scission PMID: 22157747
  4. Results describe a novel set of mutations in Sec24p that distinguish two adjacent cargo-binding sites and examine the ability of Sec22p to rescue these mutations. PMID: 20477990
Subcellular Location
Cytoplasm. Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
SEC23/SEC24 family, SEC24 subfamily
Database Links

KEGG: sce:YIL109C

STRING: 4932.YIL109C

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