Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC4 Polyclonal antibody

Uniprot No.

P07560

Target Names

SEC4

Alternative Names

SEC4 antibody; SRO6 antibody; YFL005WRas-related protein SEC4 antibody; Suppressor of RHO3 protein 6 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC4 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP-bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle.

Gene References into Functions

Data show that mutations in Rab GTPase Sec4p (SEC4) disrupt actin patch assembly and proper endocytic internalization. PMID: 27526190
Sec4p phosphorylation facilitates the coordination of membrane-trafficking events during cytokinesis. PMID: 27621363
Authors describe the identification of a Sec4 binding site on the surface of Sro7 that is contained within a cleft created by the junction of two adjacent beta-propellers that form the core structure of Sro7. PMID: 26202462
Results show that Sec4p serine 29 forms a hydrogen bond with the nucleotide and its can form stable associations with Sec2p allowing to elucidate the structure of the Sec4p-Sec2p complex representing an early stage of the exchange reaction. PMID: 26263895
Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers and highly dependent on both Sro7 and Rab GTPase, Sec4. PMID: 25404740
Data suggest that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation. PMID: 20444978
Sec2 is a highly active exchange factor, in contrast to other proteins previously characterized as Rab exchange factors. PMID: 17134721
The crystal structure of the Sec2p guanine nucleotide exchange factor domain in a complex with the nucleotide-free Sec4p at 2.7 A resolution, is reported. PMID: 17488829

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Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane; Lipid-anchor; Cytoplasmic side. Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm. Note=A small fraction is soluble.

Protein Families

Small GTPase superfamily, Rab family

Database Links

KEGG: sce:YFL005W

STRING: 4932.YFL005W

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SEC4 Antibody

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