sem-5 Antibody

Code CSB-PA341059XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans sem-5 Polyclonal antibody
Uniprot No.
Target Names
sem-5
Alternative Names
sem-5 antibody; C14F5.5 antibody; Sex muscle abnormal protein 5 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans sem-5 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Adapter protein which modulates signaling mediated by several receptor tyrosine kinases such as egl-15 and let-23 probably acting upstream of let-60/ras. Negatively regulates vulva induction probably downstream of let-23. Involved in sex myoblast migration. Negatively regulates fluid homeostasis probably downstream of egl-15. During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles probably downstream of egl-15. Involved in cytoskeleton dynamics and is recruited by mig-13 to the leading edge of Q neuroblasts and their descendants to signal downstream to activate the wsp-1 pathway and direct migration along the anteroposterior body axis during larval development. Involved in let-23-mediated regulation of fertility independently of let-60/Ras. Negatively regulates daf-2-mediated repression of dauer formation. Plays a role in nicotinic acetylcholine receptor (nAChR)-mediated sensitivity to nicotine. Regulates synaptic levels of nAchR subunit lev-1 in the nerve cord. May play a role in oocyte development upstream of let-60/Ras and the MAP kinase pathway.
Gene References into Functions
  1. MIG-13-WAVE pathway provides the major force for directional cell motility, whereas MIG-13-WASP partially compensates for its loss, underscoring their coordinated activities in facilitating robust cell migration. PMID: 27780040
  2. Solution structure, dynamics, and thermodynamics of the native state ensemble of the Sem-5 C-terminal SH3 domain. PMID: 12741814
Subcellular Location
Cell membrane.
Protein Families
GRB2/sem-5/DRK family
Tissue Specificity
Expressed in body wall muscles, pharynx, intestine and hypodermis.
Database Links

KEGG: cel:CELE_C14F5.5

STRING: 6239.C14F5.5

UniGene: Cel.19703

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