44807 Antibody

Code CSB-PA515777XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SEP3 Polyclonal antibody
Uniprot No.
Target Names
SEP3
Alternative Names
SEP3 antibody; AGL9 antibody; At1g24260 antibody; F3I6.19 antibody; Developmental protein SEPALLATA 3 antibody; Agamous-like MADS-box protein AGL9 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana 44807 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development. Binds specifically to the CArG box DNA sequence 5'-CC (A/T)6 GG-3'.
Gene References into Functions
  1. Cloning, prokaryotic expression and purification of CpfS1 (Sep3) protein from Arabidopsis thaliana has ben described. PMID: 26718440
  2. Authors analyzed the oligomeric status of SEP3 domains in the absence of the DNA-binding MADS-box domain PMID: 26505403
  3. The 2.5-A crystal structure of a small portion of the intervening and the complete keratin-like domain of SEP3, is reported. PMID: 25228343
  4. SEP3 is a member of the MADS transcription factor family and plays a role in the development of the floral organs through the formation of multiprotein complexes with other MADS-family transcription factors. PMID: 23989147
  5. Here, we show that SEP3 as a downstream gene of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (SPL3) and FT modulates the flowering time in response to different ambient temperatures. PMID: 22899051
  6. Approximately 90% of the binding sites of two well-characterized MADS domain transcription factors, APETALA1 and SEPALLATA3, were covered by the DNase I hypersensitive (DH) sites. PMID: 22773751
  7. higher-order complex formation is a general and essential molecular mechanism for plant MADS box protein functioning and SEP3 'glue' protein plays a pivotal role in mediating multimerization PMID: 19243611
  8. In order to uncover new roles of SEP3 we studied in detail the phenotypes produced by 35S::SEP3 in different wild type and mutant backgrounds. [SEPALLATA3] [SEP3] PMID: 16098111
  9. Evidence that in vitro homotetramers of the class E floral homeotic protein SEPALLATA3 from Arabidopsis thaliana bind cooperatively to two sequence elements termed 'CArG boxes' in a phase-dependent manner involving DNA looping. PMID: 19033361
  10. SEPALLATA3 integrates and modulates different growth-related and hormonal pathways in a combinatorial fashion with other MADS-box proteins and possibly with non-MADS transcription factors. PMID: 19385720

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Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT1G24260

STRING: 3702.AT1G24260.2

UniGene: At.24086

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