sepA Antibody

Code CSB-PA819796XA01SZB
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Product Details

Full Product Name
Rabbit anti-Shigella flexneri sepA Polyclonal antibody
Uniprot No.
Target Names
sepA
Alternative Names
sepA antibody; CP0070 antibody; Serine protease SepA autotransporter antibody; EC 3.4.21.-) [Cleaved into: Serine protease SepA; Serine protease SepA translocator] antibody
Raised in
Rabbit
Species Reactivity
Shigella flexneri
Immunogen
Recombinant Shigella flexneri sepA protein
Immunogen Species
Shigella flexneri
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Major protein secreted in laboratory media showing proteolytic activity. May be involved in invasion and destruction of host intestinal epithelium.
Gene References into Functions
  1. The serine protease activity of SepA protein, Shigella is required for the decrease of active LIM Kinase 1 (LIMK1) and the increase of active cofilin, a major actin-polymerization factor. The regulatory effects of SepA on LIMK1 and cofilin results in the disruption of the tight junctions at epithelial barrier during Shigella infection. PMID: 28598765
Subcellular Location
[Serine protease SepA autotransporter]: Periplasm.; [Serine protease SepA]: Secreted. Cell surface.; [Serine protease SepA translocator]: Cell outer membrane; Multi-pass membrane protein.
Database Links

KEGG: sfl:CP0070

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