serA Antibody

Code CSB-PA017920XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) serA Polyclonal antibody
Uniprot No.
Target Names
serA
Alternative Names
serA antibody; b2913 antibody; JW2880 antibody; D-3-phosphoglycerate dehydrogenase antibody; PGDH antibody; EC 1.1.1.95 antibody; 2-oxoglutarate reductase antibody; EC 1.1.1.399 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) serA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.
Gene References into Functions
  1. Data indicate that the cooperative transmission induced by serine binding is transmitted through the D-3-phosphoglycerate dehydrogenase Gly294-Gly295 hinge region to the opposite serine binding interface. PMID: 21391703
  2. L-serine, inhibitor of D-3-phosphoglycerate dehydrogenase, exerts its effect on at least two steps in the kinetic mechanism, but to very different degrees. PMID: 19924905
  3. L-Serine inhibits the catalytic activity of D-3-phosphoglycerate dehydrogenase by binding to its regulatory domain. PMID: 16363798
  4. analysis of mutation effects on effector binding and domain rotation in Escherichia coli D-3-phosphoglycerate dehydrogenase PMID: 17459882
  5. the rate-limiting step in both directions is a conformational change in the Escherichia coli D-3-phosphoglycerate dehydrogenase enzyme that is not detected optically PMID: 18776184

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Protein Families
D-isomer specific 2-hydroxyacid dehydrogenase family
Database Links
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