Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans set-2 Polyclonal antibody

Uniprot No.

Q18221

Target Names

set-2

Alternative Names

set-2 antibody; C26E6.9 antibody; Histone-lysine N-methyltransferase set-2 antibody; EC 2.1.1.- antibody; SET domain-containing protein 2 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans set-2 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Histone methyltransferase that specifically di- and trimethylates 'Lys-4' of histone H3 at all developmental stages and in adult germ cells. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Implicated in the epigenetic inheritance of lifespan over several generations. Acts in the germline to limit the longevity of the soma, probably by regulating a lipid metabolism pathway that signals from the germline to the intestine, thereby preventing accumulation of mono-unsaturated fatty acids. Methylation in the germline is required for germline development and fertility, possibly by ensuring genome stability. May act redundantly with mes-3 and mes-4 proteins in the development of a fertile germline. Required for RNAi. Functions as an antagonist of hpl-1 and hpl-2 activity in growth and somatic gonad development. Cooperates with jmjd-3.1 and egl-27 to ensure robust transdifferentiation of the Y rectal cell to the PDA motor neuron during larval development.

Gene References into Functions

Animals lacking SET-2 catalytic subunit show transgenerational increase in sensitivity to DNA damage-inducing agents that is accompanied by defect in DSB repair. These defects are not due to failure to activate the DNA damage response that allows detection, signaling and repair of DNA lesions, because cell cycle arrest and apoptosis, key components of this pathway, are efficiently induced in set2 mutants. PMID: 28779964
These results suggest that HP1 and a SET1/MLL-related complex may play antagonistic roles in the epigenetic regulation of specific developmental programs. PMID: 17967446

Subcellular Location

Nucleus. Note=Localized in mitotic and mid-late-stage meiotic nuclei but is undetectable in early pachytene nuclei.

Protein Families

Class V-like SAM-binding methyltransferase superfamily

Tissue Specificity

Expressed in all cells of embryo. In L1 larva, it is predominantly expressed in Z2 and Z3 primordial germ cells. In adults, it is predominantly expressed in the germline.

Database Links

KEGG: cel:CELE_C26E6.9

STRING: 6239.C26E6.9c.1

UniGene: Cel.8145

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set-2 Antibody

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