SET2 Antibody

1. report that both methyltransferases can be UV cross-linked to RNA in vivo High-throughput sequencing of the bound RNAs revealed strong Set1 enrichment near the transcription start site, whereas Set2 was distributed along pre-mRNAs PMID: 28483910
2. one of these mutations (R2510H), located in the Set2 Rpb1 interaction domain, did not result in an observable defect in SETD2 enzymatic function, a second mutation in the catalytic domain of this enzyme (R1625C) resulted in a complete loss of histone H3 Lys-36 trimethylation (H3K36me3). PMID: 27528607
3. Set2 suppresses the restoration of unstable ON telomeres to the stable OFF state and promotes cellular aging. PMID: 24607280
4. Set2 functions both to suppress the incorporation of acetylated histones and to signal for the deacetylation of these histones in transcribed genes. By suppressing spurious cryptic transcripts from initiating within ORFs, this pathway is essential to maintain the accuracy of transcription by RNA polymerase II. PMID: 22914091
5. RNA polymerase II carboxyl-terminal domain phosphorylation regulates protein stability of the Set2 methyltransferase and histone H3 di- and trimethylation at lysine 36. PMID: 22157004
6. Mapping of interaction domains revealed the importance of the SET core domain which is necessary and sufficient for binding Ino2. PMID: 20054697
7. Asf1 stimulates Set2 occupancy of the coding region of a highly transcribed gene by a mechanism that depends on Asf1 binding to H3/H4. PMID: 20048053
8. A novel domain in Set2 has been defined, which interacts with RNA polymerase II and is responsible for the methylation of histone 3 at K36 required for the transcription elongation process. PMID: 15798214
9. The solution structure of the yeast Set2 SRI domain reveals a novel carboxyl-terminal domain binding fold consisting of a left-handed three-helix bundle PMID: 16286474
10. SGV1 is required for the normal pattern of histone methylation by SET2. PMID: 16581778
11. yFACT and Set2 oppose one another during transcriptional initiation at a step involving DNA binding by TBP and TFIIA. PMID: 16977311
12. S-phase progression is suppressed by set2 or chd1 mutations, suggesting that Set2 and Chd1 have specific roles in negatively regulating DNA replication. PMID: 18245327
13. Loss of Set2p or Rpd3p substantially elevated HIS4 hotspot activity, and loss of Hda1p had a smaller stimulatory effect PMID: 18515193
14. Results suggest that suppression of cryptic transcription via the Set2/Rpd3S pathway occurs independent of gene length or transcriptional frequency. PMID: 19295910
15. Data suggest that Set2 is controlled by intratail interactions which can be influenced by modifications and changes to the structure of the H3 tail to control the dynamics and localization of methylation during elongation. PMID: 19822661

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KEGG: sce:YJL168C

STRING: 4932.YJL168C