setdb2 Antibody

Code CSB-PA117895XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) setdb2 Polyclonal antibody
Uniprot No.
Target Names
setdb2
Alternative Names
setdb2 antibody; zgc:77298 antibody; Histone-lysine N-methyltransferase SETDB2 antibody; EC 2.1.1.- antibody; SET domain bifurcated 2 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) setdb2 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. During early development, required to specify the left-right axis by repressing expression of FGF8, leading to negatively regulate the dorsal organizer formation.
Gene References into Functions
  1. Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1. PMID: 24892953
  2. Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. PMID: 20133783
Subcellular Location
Nucleus. Chromosome.
Protein Families
Class V-like SAM-binding methyltransferase superfamily
Database Links

KEGG: dre:335153

UniGene: Dr.82071

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