SAG101 Antibody

Code CSB-PA214050XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SAG101 Polyclonal antibody
Uniprot No.
Target Names
SAG101
Alternative Names
SAG101 antibody; At5g14930 antibody; F2G14.50 antibody; Senescence-associated carboxylesterase 101 antibody; EC 3.1.1.1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SAG101 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Acyl hydrolase that triggers the leaf senescence onset. Can use triolein as substrate to produce oleic acids.; Involved in the EDS1-dependent intrinsic and indispensable resistance signaling pathway; together with PAD4, required for programmed cell death triggered by RPS4 in response to avirulent pathogens (e.g. P.syringae pv. tomato strain DC3000 and H.parasitica isolates CALA2 and EMWA1) and in restricting the growth of virulent pathogens (e.g. H.parasitica isolates NOCO2 and P.syringae pv. tomato strain DC3000 avrRps4). Contributes in reinforcing the immune response around hypersensitive response foci. Regulates the nuclear localization of EDS1. Essential for the RPP8/HRT-mediated resistance to the turnip crinkle virus (TCV). Involved in the post-invasion resistance to P.pachyrhizi in the mesophyll.
Gene References into Functions
  1. SAG101 is required for R-mediated resistance to TCV, thus implicating a role for the ternary complex in this defense response. PMID: 22072959
Subcellular Location
Membrane; Single-pass membrane protein. Nucleus. Cytoplasm. Note=Can move to the cytoplasm when in complex with PAD4 and EDS1.
Tissue Specificity
Expressed in senescing leaves.
Database Links

KEGG: ath:AT5G14930

STRING: 3702.AT5G14930.2

UniGene: At.23431

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