Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SGO1 Polyclonal antibody

Uniprot No.

Q08490

Target Names

SGO1

Alternative Names

SGO1 antibody; YOR073W antibody; YOR29-24 antibody; Shugoshin antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SGO1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plays a central role in chromosome cohesion during mitosis and meiosis divisions by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. Probably act by protecting REC8 and RAD21 from separase degradation during anaphase. Also acts as a spindle checkpoint component required for sensing tension between sister chromatids during mitosis, its degradation when they separate preventing cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.

Gene References into Functions

The TSM-Sgo1p-cohesin triad is therefore at the center stage of higher-ordered chromatin architecture for error-free segregation. PMID: 30002083
sgo1 mutants with abolished binding to H2A or PP2A(Rts1) displayed premature SAC silencing. PMID: 28040741
Sgo1 operates as a multifunctional hub that coordinates two centromeric functions essential for correct chromosome segregation. PMID: 24945276
budding yeast shugoshin Sgo1 dissociates from the pericentromere reversibly in response to tension PMID: 24939933
Sister kinetochores are frequently bi-oriented in the absence of chiasmata and that Sgo1 prevents equational segregation of sister chromatids during achiasmate meiosis I. PMID: 21330786
Sgo1 is required for sensing tension between sister chromatids during mitosis, and its degradation when they separate may prevent cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension PMID: 15637284
Sgo1 is required for protecting centromeric cohesins from removal during meiosis I, localizes to cohesin-associated regions (CARs) at the centromere PMID: 16357219
Sgo1 acts at sister kinetochores to promote their biorientation. PMID: 18094053
Report structure and function of the PP2A-shugoshin interaction. PMID: 19716788

Hide All

Subcellular Location

Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole.

Protein Families

Shugoshin family

Database Links

KEGG: sce:YOR073W

STRING: 4932.YOR073W

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SGO1 Antibody

Product Details

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Target Background