SGS3 Antibody

Code CSB-PA878471XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SGS3 Polyclonal antibody
Uniprot No.
Target Names
SGS3
Alternative Names
SGS3 antibody; At5g23570 antibody; MQM1.17 antibody; Protein SUPPRESSOR OF GENE SILENCING 3 antibody; AtSGS3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SGS3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for post-transcriptional gene silencing and natural virus resistance. May bind nucleic acids and is essential for the biogenesis of trans-acting siRNAs but is not required for silencing induced by IR-PTGS. Involved in the juvenile-to-adult transition regulation. In case of begomoviruses infection, it is targeted by the viral protein V2 leading to suppression of post-transcriptional gene silencing.
Gene References into Functions
  1. Viral genome-linked protein (VPg) acts as a suppressor of RNA silencing and suggest that an interaction with SGS3 may be important, especially in suppression of sense-mediated RNA silencing. PMID: 25099340
  2. Temperature increase reduces the protein abundance of SUPPRESSOR OF GENE SILENCING 3 and induce transgenerational epigenetic release of RNA silencing by inhibiting siRNA biogenesis in Arabidopsis. PMID: 23686579
  3. SGS3 associates with AGO1-RISC via the double-stranded RNA formed by the 3'-terminal nucleotides of 22-nt miR173 and corresponding target RNA, which probably protrudes from the AGO1-RISC molecular surface PMID: 23417299
  4. Components of the RNA-silencing machinery, RNA-DEPENDENT RNA POLYMERASE1 and SUPPRESSOR OF GENE SILENCING3 were isolated, implicating RNA silencing in the control of cuticular wax deposition during inflorescence stem development in Arabidopsis . PMID: 22689894
  5. Null sgs3 mutants lack TAS1, TAS2, and TAS3 ta-siRNAs and overaccumulate ARF3/ETTIN and ARF4 mRNAs, which are TAS3 ta-siRNA targets. PMID: 16682354
  6. sgs3-3 is a neomorphic allele that shows increased RNA protective activity PMID: 19143842

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Subcellular Location
Cytoplasm, perinuclear region.
Protein Families
SGS3 family
Database Links

KEGG: ath:AT5G23570

STRING: 3702.AT5G23570.1

UniGene: At.31001

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