SHT Antibody

Code CSB-PA199855XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SHT Polyclonal antibody
Uniprot No.
Target Names
SHT
Alternative Names
SHT antibody; At2g19070 antibody; T20K24.8 antibody; Spermidine hydroxycinnamoyl transferase antibody; EC 2.3.1.- antibody; BAHD-like hydroxycinnamoyl transferase antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SHT protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Hydroxycinnamoyl transferase involved in the conjugation of feruloyl CoA to spermidine. Catalyzes the three conjugating steps required for the biosynthesis of N(1),N(4),N(8)-triferuloyl-spermidine. Spermidine is the only acceptor substrate while feruloyl CoA > caffeoyl CoA > coumaroyl CoA > cinnamoyl CoA >> sinapoyl CoA are efficient acyl donors. No activity with hydroxyferuloyl CoA. Required for the biosynthesis of these conjugated spermidine derivatives, specifically in anther tapetum.
Gene References into Functions
  1. SHT is specifically expressed in anther tapetum cells in the early stages of flower development, and involved in the synthesis of hydroxycinnamoyl spermidines. PMID: 19077165
Protein Families
Plant acyltransferase family
Tissue Specificity
Expressed in flower buds and inflorescences. Restricted to anther tapetum.
Database Links

KEGG: ath:AT2G19070

STRING: 3702.AT2G19070.1

UniGene: At.27755

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