Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans sid-1 Polyclonal antibody

Uniprot No.

Q9GZC8

Target Names

sid-1

Alternative Names

sid-1 antibody; C04F5.1 antibody; Systemic RNA interference defective protein 1 antibody; Systemic RNAi enabling protein antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans sid-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays a role in RNA-mediated gene silencing by acting cell-autonomously as a channel for the transport of double-stranded RNA (dsRNA) between cells. Mediates the spread of dsRNA and subsequent silencing of genes in cells distant from the site of dsRNA introduction. Selective for dsRNA. Preferentially binds long dsRNA, from 50 base pairs up to 700. Short 20 base-pair long molecules are not bound. May also bind dsDNA, but with lower affinity. Binding may be sequence-independent. Required for avoidance behavior induced by small RNAs derived from pathogenic bacteria such as P.aeruginosa.

Gene References into Functions

consistent with previous studies, we fail to detect a requirement for sid-1 in dsRNA export, but demonstrate for the first time that SID-1 functions in the intestine to support environmental RNAi (eRNAi). PMID: 29025917
sid-1 is required for inheritance of silencing RNAs. dsRNA transit through the endocytosis pathway in early embryos is independent of sid-1. PMID: 28751423
Epigenetic silencing of the endogenous sid-1 locus is maintained by germline RNAi-mediated inheritance for up to 13 generations. PMID: 28212751
The functional importance of SID-1 extracellular domain as a dsRNA binding domain that contributes to RNA transport. PMID: 26067272
SID-1 is a dsRNA-selective dsRNA-gated channel. PMID: 21474576
C. elegans expressing neuronal SID-1 showed RNAi phenotypes when fed with bacteria expressing dsRNA for known neuronal genes and for uncharacterized genes with no previously known neuronal phenotypes. PMID: 20512143
data indicate that systemic RNA interference in C. elegans involves SID-1-mediated intercellular transport of dsRNA PMID: 12970568
Study shows that equivalent masses of long or short dsRNA accumulate in Drosophila S2 cells, indicating that size-dependent silencing is not due to size-selective transport through SID-1. PMID: 19155320
C. elegans can transport endogenous and exogenous RNA silencing signals between many different tissues via at least 2 SID-1 independent export pathways PMID: 19168628

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Note=Enriched at the cell periphery and also to the punctate structures of cytoplasm.

Protein Families

SID1 family

Tissue Specificity

Expressed in most non-neuronal cells, including body wall muscle cells.

Database Links

KEGG: cel:CELE_C04F5.1

STRING: 6239.C04F5.1

UniGene: Cel.179

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sid-1 Antibody

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