SIGB Antibody

^{Rare Species}

Datasheet

Code	CSB-PA788836XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: SIGB Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

O22056

Target Names

SIGB

Alternative Names

RNA polymerase sigma factor sigB (Sigma factor B) (Sigma-B) (Protein ABERRANT CHLOROPLAST 1) (RNA polymerase sigma factor sig1) (Atsig1) (Sigma factor 1) (RNA polymerase sigma factor sig2) (Atsig2) (Sigma factor 2) (RNA polymerase sigma factor sigA) (Sigma factor A) (Sigma-A) SIGB ABC1 SIG1 SIG2 SIGA At1g08540 F22O13.2 T27G7.22

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SIGB protein (40-310aa)

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To produce the SIGB polyclonal antibody, a recombinant Arabidopsis thaliana SIGB protein segment (amino acids 40-310) is used as the immunogen to stimulate an antibody response in a rabbit. The serum collected from the rabbit contains polyclonal antibodies, which are then purified using affinity chromatography. The proficiency of the SIGB antibody in detecting the Arabidopsis thaliana SIGB protein is confirmed through ELISA and WB assays, ensuring its effectiveness in experimental conditions.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Required for the transition of plastids into chloroplasts by coordinating nuclear and chloroplastic genomes under light conditions. Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released. Promotes the biosynthesis of plastid-encoded tRNAs (e.g. trnE-UUC and trnV-UAC).

Gene References into Functions

Phytochrome-induced SIG2 expression contributes to photoregulation of phytochrome signalling and photomorphogenesis in Arabidopsis thaliana. PMID: 24078666

Subcellular Location

Plastid, chloroplast.

Protein Families

Sigma-70 factor family

Tissue Specificity

Highly expressed in cotyledons, to a lesser extent in leaves, sepals and siliques, and barely expressed in roots. Present in seedlings.

Database Links

KEGG: ath:AT1G08540

STRING: 3702.AT1G08540.1

UniGene: At.23349

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Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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