SIGB Antibody

Rare Species
Code CSB-PA788836XA01DOA
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes: SIGB Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
Promotion Free Antibody trial simple
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Product Details

Uniprot No.
Target Names
SIGB
Alternative Names
RNA polymerase sigma factor sigB (Sigma factor B) (Sigma-B) (Protein ABERRANT CHLOROPLAST 1) (RNA polymerase sigma factor sig1) (Atsig1) (Sigma factor 1) (RNA polymerase sigma factor sig2) (Atsig2) (Sigma factor 2) (RNA polymerase sigma factor sigA) (Sigma factor A) (Sigma-A) SIGB ABC1 SIG1 SIG2 SIGA At1g08540 F22O13.2 T27G7.22
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SIGB protein (40-310aa)
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To produce the SIGB polyclonal antibody, a recombinant Arabidopsis thaliana SIGB protein segment (amino acids 40-310) is used as the immunogen to stimulate an antibody response in a rabbit. The serum collected from the rabbit contains polyclonal antibodies, which are then purified using affinity chromatography. The proficiency of the SIGB antibody in detecting the Arabidopsis thaliana SIGB protein is confirmed through ELISA and WB assays, ensuring its effectiveness in experimental conditions.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Required for the transition of plastids into chloroplasts by coordinating nuclear and chloroplastic genomes under light conditions. Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites and are then released. Promotes the biosynthesis of plastid-encoded tRNAs (e.g. trnE-UUC and trnV-UAC).
Gene References into Functions
  1. Phytochrome-induced SIG2 expression contributes to photoregulation of phytochrome signalling and photomorphogenesis in Arabidopsis thaliana. PMID: 24078666
Subcellular Location
Plastid, chloroplast.
Protein Families
Sigma-70 factor family
Tissue Specificity
Highly expressed in cotyledons, to a lesser extent in leaves, sepals and siliques, and barely expressed in roots. Present in seedlings.
Database Links

KEGG: ath:AT1G08540

STRING: 3702.AT1G08540.1

UniGene: At.23349

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