Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SIGE Polyclonal antibody

Uniprot No.

Q9ZNX9

Target Names

SIGE

Alternative Names

SIGE antibody; SIG5 antibody; At5g24120 antibody; MLE8.4 antibody; RNA polymerase sigma factor sigE antibody; chloroplastic/mitochondrial antibody; Sigma factor E antibody; Sigma-E antibody; RNA polymerase sigma factor sig5 antibody; Atsig5 antibody; Sigma factor 5 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SIGE protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential protein. Sigma factors are initiation factors that promote the attachment of plastid-encoded RNA polymerase (PEP) to specific initiation sites (e.g. psbA and psbD) and are then released. Essential for blue light-mediated transcription of psbD, which encodes the photosystem II reaction center protein D2. Plays a role in reproduction. Required during female gametophyte development.

Gene References into Functions

the circadian regulation of chloroplast transcription by SIG5 was predominantly dependent on blue light and cryptochrome. PMID: 27716936
The role of SIG5 protein in the transcription of chloroplast genes via protein phosphatase 2C-dependent activation of nuclear genes PMID: 25976841
nuclear-encoded SIGMA FACTOR5 (SIG5) controls circadian rhythms of transcription of several chloroplast genes, revealing one pathway by which the nuclear-encoded circadian oscillator controls rhythms of chloroplast gene expression. PMID: 23493713
The NEP (the (phage-type) plastid RNA polymerase) promoter cluster can help maintain RNA synthesis in situations where no functional sigma factor is available for PEP (the bacterial-type plastid RNA polymerase). PMID: 17118361
Cryptochromes 1 and 2 mediate SIG5 transcription at different light wavelengths. SIG5 activates the psbD blue-light-responsive promoter. PMID: 18532976

Hide All

Subcellular Location

[Isoform 1]: Plastid, chloroplast.; [Isoform 2]: Mitochondrion.

Protein Families

Sigma-70 factor family

Tissue Specificity

Mostly expressed in siliques and flowers, to a lesser extent in leaves and stems, and barely expressed in roots (at protein level). Isoform 2 accumulates specifically in flowers, whereas isoform 1 is expressed in both leaves and flowers. Present in old se

Database Links

KEGG: ath:AT5G24120

STRING: 3702.AT5G24120.1

UniGene: At.5425

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SIGE Antibody

Product Details

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Target Background