SINAT5 Antibody

Datasheet

Code	CSB-PA837303XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SINAT5 Polyclonal antibody

Uniprot No.

Q8S3N1

Target Names

SINAT5

Alternative Names

SINAT5 antibody; At5g53360 antibody; K19E1.16 antibody; E3 ubiquitin-protein ligase SINAT5 antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase SINAT5 antibody; Seven in absentia homolog 5 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SINAT5 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and proteasomal-dependent degradation of NAC021/NAC022, a transcription activator that functions downstream of the auxin signals, thereby acting as a down-regulator of auxin signals. Involved in the formation of lateral roots. Is antagonist to SINAT1, SINAT2, SINAT3 and SINAT4 by suppressing FREE1 ubiquitination and degradation mediated by SINAT1, SINAT2, SINAT3 and SINAT4, and promoting FREE1 accumulation.

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

SINA (Seven in absentia) family

Tissue Specificity

Expressed at low level in the vascular tissue of mature roots. Expressed in lateral roots and in elongation zone of the main root upon stimulation by auxin. Colocalizes with NAC021/NAC022.

Database Links

KEGG: ath:AT5G53360

STRING: 3702.AT5G53360.1

UniGene: At.24561

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SINAT5 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background